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. 2009 Feb 17;77(5):1746–1756. doi: 10.1128/IAI.01530-08

FIG. 5.

FIG. 5.

Two-dimensional SDS-PAGE and Western blot analyses of A. phagocytophilum NCH-1A Msp2(P25) proteins. Hydrophilic pellet fractions enriched for A. phagocytophilum NCH-1A outer membrane proteins were isoelectric focused (IEF) in IPG strips (pH 5.0 to 8.0), which was followed by resolution in the second dimension in SDS-polyacrylamide (4 to 20%) gels. (A) Silver-stained gel. Msp2(P25) proteins that were excised for LC-MS/MS identification are indicated by a box. (B) Enlarged view of the region of the silver-stained gel in panel A indicated by the box. Numbered spots were excised and identified by LC-MS/MS. (C) MAb 20B4 recognized spots in the predicted size range for full-length Msp2(P44) and truncated Msp2(P25) proteins.