TABLE 4.

Msp2(P44) paralogs expressed by NCH-1, NCH-1A, and NCH-1A2^a

Paralogb	The Institute for Genomic Research designation	GenBank accession no.c	% Identityd	% of clones
NCH-1 in HL-60 cells
(P44)-4	APH_1154	AAL86382	96.6-98	2.6
(P44)-18	APH_1194	AAQ84091	100	2.6
(P44)-20	APH_1390	AAL86394	99.3	2.6
(P44)-23	APH_1256	YP_505777	94.3-99.3	89.5
(P44)-56	APH_1078	AAP68670	98.5	2.6
NCH-1A in HL-60 sLex(−/low) cells
(P44)-4	APH_1154	AAL86382	90.5-100	75.0
(P44)-6	APH_1121	AAL86384	100	3.1
(P44)-11	APH_1392	YP_505888	99.3-100	6.2
(P44)-12	APH_1249	YP_505773	100	3.1
(P44)-43	APH_1184	AAO59315	100	3.1
(P44)-62	APH_1018	AAQ75552	94.5	6.2
(P44)-c20	NAe	AAP20824	99.2	3.1
NCH-1A in HL-60 cells
(P44)-4	APH_1154	AAL86382	96.6	42.0
(P44)-5	APH_1312	AAL86383	100	4.0
(P44)-6	APH_1121	AAL86384	100	2.0
(P44)-13	APH_1238	AAL86390, AAP14029	99.2-100	22.0
(P44)-16	APH_1269	YP_505788	99.3	2.0
(P44)-18	APH_1194	AAQ84091	100	2.0
(P44)-19	APH_1169	YP_505708, ABF81554	68.0-76.7	4.0
(P44)-24	APH_1311	YP_505824	100	10.0
(P44)-43	APH_1184	AAO59315	100	4.0
(P44)-50	APH_1080	AAP32153, AAL78190	67.1-100	6.0
(P44)-53	APH_1155	AAP70037	100	2.0
NCH-1A2 in HL-60 A2 cells
(P44)-18	APH_1194	AAQ84091	98.5-99.3	96.6
(P44)-24	APH_1311	YP_505824	100	1.7
(P44)-c20	NA	AAP20824	98.5	1.7

^acDNAs corresponding to the HVRs of msp2(p44) cDNAs were TA cloned and sequenced. The nucleotide sequences were translated into amino acid sequences, which were utilized in BLAST searches.

^bBold type indicates paralogs corresponding to the majority of sequenced clones and data for these paralogs.

^cThe paralog nomenclature is the nomenclature used in the original The Institute for Genomic Research or GenBank entries. The Institute for Genomic Research designations correspond to designations for Msp2(P44) paralogs listed in the annotated A. phagocytophilum HZ genome.

^dLevels of amino acid identity for translated msp2(p44) HVR RT-PCR clone sequences with The Institute for Genomic Research and GenBank Msp2(P44) paralog entries.

^eNA, not applicable.