TABLE 5.

Msp2 (P44) paralogs expressed by NCH-1 and NCH-1A2 after 100 passages^a

Paralogb	The Institute for Genomic Research designationc,d	GenBank accession no.c	% Identitye	% of clones
NCH-1 in HL-60 cells
(P44)-6	APH_1121	AF412821	99.7	1.7
(P44)-9	APH_1391	AF412824	99.3	1.7
(P44)-13	APH_1238	AY763489	99.8	1.7
(P44)-23	APH_1256	YP_505777	81.7-95	91.5
(P44)-59	APH_1408	AY279321	98.9	1.7
(P44)-62	APH_1018	AY307376	95	1.7
NCH-1A2 in HL-60 A2 cells
(P44)-18	APH_1194	AAQ84091	97.7-100.0	85.0
(P44)-24	APH_1311	AY763480	100	1.7
(P44)-44	APH_0981	AY147265	94.5-94.7	1.7
(P44)-47	APH_1152	AY147264	93.7	1.7
(P44)-48	APH_1272	AY147267	73.7-75.5	3.3
(P44)-59	APH_1408	AY279321	71.4	1.7
(P44)-62	APH_1018	AAP20824	98.5	5.0

^aNCH-1 and NCH-1A2 were cultivated in HL-60 and HL-60 A2 cells, respectively, for 100 passages from the point at which their Msp2(P44) expression profiles were examined to obtain the data shown in Table 4. cDNAs corresponding to the HVRs of msp2(p44) cDNAs were TA cloned and sequenced. The nucleotide sequences were translated into amino acid sequences, which were utilized in BLAST searches.

^bBold type indicates paralogs corresponding to the majority of sequenced clones and data for these paralogs.

^cThe paralog nomenclature is the nomenclature used in the original The Institute for Genomic Research or GenBank entries.

^dThe Institute for Genomic Research designations correspond to designations for Msp2(P44) paralogs listed for the annotated A. phagocytophilum HZ genome.

^eLevels of amino acid identity for translated msp2(p44) HVR RT-PCR clone sequences with The Institute for Genomic Research and GenBank MSP2(P44) paralog entries.