FIG. 4.

High extracellular potassium levels inhibit Asc-dependent caspase-1 activation. An immunoblot of the p10 fragment of caspase-1 from combined supernatants and pellets of BMMs from wild-type (WT), Asc^−/−, or Ipaf^−/− mice is shown. Cells were infected for 4 h with wild-type, dotA, or flaA L. pneumophila at an MOI of 10 in the presence of an additional 50 mM NaCl or 50 mM KCl. Data are representative of three experiments.