TABLE 3.
Forty-one S. aureus isolates from multiple specimens from selected patients with chronic or acute osteomyelitisa
| Osteomyelitis and strain | Sample | α-Hemolysin | β-Hemolysin | CP serotype | cap allele | Slime productione | Susceptibility to methicillinf | Pulsotypeb | spa type |
|---|---|---|---|---|---|---|---|---|---|
| Chronic | |||||||||
| 85a | Bone (purulent secretion) | + | + | CP8 | cap8 | + | S | D | t189 |
| 85b | Bone (tibia, distal) | + | + | CP5 | cap5 | + | R | A9 | t149 |
| 85c | Fibrous soft tissue | − | − | NT | cap8 | + | S | D | t189 |
| 85d | Bone (tibia, proximal) | + | + | NT | cap5 | + | R | A9 | t149 |
| 85e | Bone (transplant) | − | − | NT | cap8 | + | S | D | t189 |
| 85f | Bone (tibia) | − | − | NT | cap8 | + | S | D | t189 |
| Chronic | |||||||||
| 92a | Soft tissue adjacent to bone | − | − | CP8 | cap8 | + | S | C | t065 |
| 92b | Cranial bone | + | + | CP8 | cap8 | + | S | C | t065 |
| 92c | Cranial plate | − | − | CP8 | cap8 | ++ | S | C | t2271 |
| Chronic | |||||||||
| 94a | Hip bone | + | + | CP5 | cap5 | +++ | R | A7 | t149 |
| 94b | Bone cement | + | + | CP5 | cap5 | +++ | R | A7 | t149 |
| 94c | Soft tissue adjacent to bone | + | − | CP5 | cap5 | +++ | R | A7 | t149 |
| 94d | Fistulous purulent secretion | − | − | NT | cap5 | +++ | R | A7 | t149 |
| Chronic | |||||||||
| 95a | Articular fluid | + | + | NT | cap8 | + | S | E1 | t2271 |
| 95b | Bone (astragalus) | + | + | NT | cap8 | + | S | E1 | t2271 |
| 95c | Bone (tibia) | + | + | NT | cap8 | + | S | E1 | t2271 |
| Chronic | |||||||||
| 107a | Bone cement | − | − | CP5 | cap5 | − | R | A2 | t3946 |
| 107a1c | Bone cement | − | − | CP5 | cap5 | +++ | R | A3 | t149 |
| 107b | Bone (femur) | + | + | CP5 | cap5 | − | R | A8 | t149 |
| 107c | Soft tissue adjacent to bone | − | − | NT | cap5 | +++ | R | A5 | Undefinedd |
| 107d | Hip purulent secretion | − | − | CP5 | cap5 | +++ | R | A4 | t149 |
| 107e | Cotyloid cement | + | + | CP5 | cap5 | + | R | A4 | t149 |
| 107f | Cotyloid membrane | − | − | CP5 | cap5 | +++ | R | A6 | t149 |
| Chronic | |||||||||
| 110a | Bone membrane (tibia) | − | − | NT | cap8 | +++ | S | E2 | t3796 |
| 110b | Bone membrane (femur) | − | − | NT | cap8 | +++ | S | E3 | t3796 |
| 110c | Bone cement | − | − | NT | cap8 | +++ | S | E3 | t3796 |
| 110d | Bone (femur) | − | − | NT | cap8 | +++ | S | E3 | t3796 |
| Acute | |||||||||
| 100a | Hip purulent aspirate | + | + | CP5 | cap5 | + | S | B | t002 |
| 100b | Cotyloid membrane | + | + | CP5 | cap5 | + | S | B | NDg |
| 100c | Bone cement | + | + | CP5 | cap5 | + | S | B | ND |
| 100d | Soft tissue adjacent to bone | + | + | CP5 | cap5 | + | S | B | ND |
| Acute | |||||||||
| 111a | Superficial secretion | + | + | CP5 | cap5 | + | R | A1 | t311 |
| 111b | Superficial secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
| 111c | Superficial secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
| 111d | Superficial secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
| 111e | Superficial secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
| 111f | Foot secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
| 111g | Deep secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
| 111h | Deep secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
| 111i | Bone (tibia) secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
| 111j | Bone (fibula) secretion | + | + | CP5 | cap5 | + | R | A1 | ND |
a
All of the strains produced positive PCR amplification for sequences specific for S. aureus and positive amplification for nuc. These 41 isolates are different from the 99 described in Table 2.
b
Pulsotype denomination arbitrarily defined for this paper.
c
Isolate 107a-1 exhibited the SCV phenotype.
d
DNA extracted from strain 107c did not yield any PCR amplification product with the primers routinely used for spa. It did, however, yield PCR amplification of specific S. aureus sequences (17).
e
Levels of slime production: −, absent; +, light; ++, moderate; +++, heavy.
f
S, susceptible; R, resistant.
g
ND, not done.