FIG. 2.

(a) Antibody responses of mice injected with plasmid pVax-FcBoNT/A or pVax-Fc*BoNT/A, with subsequent electrotransfer (b). Antibody responses were measured in mice that were injected only (Fc*BoNT/A) or injected followed by electrotransfer (Fc*BoNT/A+ET) with plasmid pVax-Fc*BoNT/A. Swiss mice (n = 4) were treated with 40 μg of plasmid DNA. Antibody titers were determined by ELISA on serum samples at different time points after treatment. Results show means ± SEM. ***, P < 0.001; #, the titer is below the limit of detection.