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. 2009 Feb 23;77(5):2221–2229. doi: 10.1128/IAI.01269-08

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FIG. 6. — (a) Antibody responses of mice injected with plasmid pVax-Fc*BoNT/A or pVax-SecEpo-Fc*BoNT/A, with electrotransfer, at day 0 and boosted with a recombinant protein injection (prot.) at day 60. Swiss mice (n = 4) were treated with 40 μg of plasmid DNA and 1 μg of recombinant FcBoNT/A homogenized in 1 mg aluminum hydroxide. Antibody titers were determined by ELISA on serum samples at different time points after treatment. Results show means ± SEM. ***, P < 0.001 (significant difference between day 70 and previous titers). (b) Antibody responses of mice injected with plasmid pVax-SecEpo-Fc*BoNT/B with electrotransfer at day 0 and boosted with a recombinant protein injection at day 30. Swiss mice (n = 4) were treated with 40 μg of plasmid DNA and 1 μg of recombinant FcBoNT/B homogenized in 1 mg aluminum hydroxide. Antibody titers were determined by ELISA on serum samples at different time points after treatment. Results show means ± SEM.