TABLE 1.
Oligonucleotides used in this study
| Primer | Sequencea | Restriction enzyme site |
|---|---|---|
| Hfq-1 | attcagaattcGGTTTCCGTGCGGGTGGTAAGGC | EcoRI |
| Hfq-2 | GCTAAAGGACAAATGTTGCAggatccGCACGAAGCATGACGTGTC | BamHI |
| Hfq-3 | GACACGTGATGCTTCGTGCggatccTGCAACATTTGTCCTTTAGC | BamHI |
| Hfq-4 | attcagaagcttACGCGAAGCAGGCAGGTCTATGG | HindIII |
| Hfq-F | attcagcatATGACAGCTAAAGGACAAATGTTGCAAG | NdeI |
| Hfq-R | attcagctcgagTTATTCGGCAGGCTGCTGGACGGTTTCC | XhoI |
| Hfq-R-Ns | attcagatgcatTTATTCGGCAGGCTGCTGGACGGTTTCC | NsiI |
| Hfq-S1 | CTCAGGAGAACAACGTATTGATCG | |
| Hfq-S2 | GTTACCATCGTATTGCTGAAtTCGCCCACATC | EcoRI |
| 0747-F | GAAAGTGTGGGCATTCGAC | |
| 0747-R | TTGCTGTAACCTGCTTCCTG | |
| 16S_F | ACGGAGGGTGCGAGCGTTAATC | |
| 16S_R | CTGCCTTCGCCTTCGGTATTCCT |
a
Capital letters in roman type indicate N. meningitidis-derived sequences, capital letters in italics indicate E. coli-derived sequences, lowercase letters indicate sequences added for cloning purposes, and underlining indicates recognition sites.