TABLE 1.
Bacterial strains and plasmids used in this study
| Plasmid or strain | Relevant characteristicsa | Source or reference |
|---|---|---|
| Plasmids | ||
| pGP704 | oriR6K mobRP4 bla | 40 |
| pHP45Ω | ori(pMB1) bla; Strepr | 46 |
| pRDH10 | oriR6K mobRP4 cat sacRB; Tcr | 28 |
| pSPN29 | oriR6K mobRP4 cat sacRB, upstream and downstream regions of fliC | This study |
| pSW127 | oriR6K mobRP4 bla, internal fragment of invA | This study |
| Strains | ||
| E. coli | ||
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 lacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| CC118 λpir | araD139 Δ(ara leu)7697 ΔlacX74 phoA20 galE galK thi rpsE rpoB argEam recA1 λpir | 22 |
| DH5α λpir | F−endA1 hsdR17 (r− m+) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U189 φ80lacZΔM15 λpir | 44 |
| S17-1 λpir | C600::RP4 2-(tet::Mu) (kan::Tn7) λpir recA1 thi pro hsdR (r− m+) | 61 |
| S. enterica serovar Typhimurium | ||
| LT2 | Laboratory strain | ATCC 700720 |
| IR715 | Fully virulent spontaneous nalidixic acid-resistant derivative of serotype Typhimurium strain ATCC 14028 | 66 |
| EHW26 | IR715 fliC::Tn10 fljB5001::MudJ | 48 |
| SPN286 | IR715 fliC5050::MudJ | This study |
| SPN287 | IR715 fljB5001::MudCm | This study |
| SPN303 | IR715 ΔfliC(−25 to +1494) | This study |
| SPN313 | IR715 ΔfliC(−25 to +1494) fljB5001::MudCm | This study |
| SPN315 | IR715 flgK5396::MudJ ΔfliC(−25 to +1494) fljB5001::MudCm | This study |
| SW215 | IR715 flgK5396::MudJ | 76 |
| SW358 | IR715 flgK5396::MudJ ΔfliC(−25 to +1494) fljB5001::MudCm invA::pSW127 | This study |
| SW399 | IR715 invA::pSW127 | This study |
| SW473 | IR715 ΔfliC(−25 to +1494) fljB5001::MudJ | This study |
| TH4881 | LT2 pNK2880(Carbr, Tn10 tnpA[ats-1 ats-2]) fliC5050::MudJ fljB5001::MudCm | 51 |
| TH5507 | LT2 flgK5396::MudJ | 3 |
a
Carbr, carbenicillin resistance; Tcr, tetracycline resistance; Specr, spectinomycin resistance.