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. 2009 Mar 6;191(9):2993–3002. doi: 10.1128/JB.01156-08

FIG. 2.

FIG. 2.

The hcnA promoter is controlled by AlgZ and AlgR. (A) S1 nuclease protection assay of the hcnA promoter. Previously mapped T1 and T2 transcriptional start sites (indicated by respective labels and arrows) of the nonmucoid strain PAO1 and ΔalgR and ΔalgZ derivatives. The open triangle with the adjacent black line denotes the beginning of the common deletion region found upstream of the hcnA promoter. (B) S1 nuclease protection assay identifying T3 transcriptional start site. The arrow indicates the band of protection indicating the +1 start site. An asterisk denotes the first nucleotide in the antisense probe. (C) Sequence of the hcnA promoter region. The shaded sequence denotes the exoY deletion region. Binding sites are underlined for AlgR (AlgR Binding Site), ANR (ANR Box), RhlR and LasR (lux α and lux β), and ribosome (RBS). +1, HcnA translational start site; boldface nucleotides, hcnA open reading frame.