FIG. 3.
Strains with the exoY chromosomal deletion region upstream of hcnA produced decreased amounts of cyanide. (A) HCN production from nonmucoid wild-type PAO1 and laboratory strains with the exoY deletion region (PA14, PA103, and PAK), the PAK algR deletion (PAK algR::Gm), the PAK mucA22 mutation, the PAK mucA22 algR deletion, the PAK pscC deletion (PAK ΔpscC), and the PAK pscC deletion with the PAO1 hcnA promoter (PAK HCN KI). (B) HCN produced from PAO1, CF isolate CF27, eye isolate 19660, urinary tract infection isolate S54485, and blood isolate X13273. Statistical significance was determined by one-way ANOVA and the Tukey-Kramer test. **, P < 0.01, and ***, P < 0.001, compared to the isogenic parent.