TABLE 1.
Strains used in this study
| Strain or plasmid | Description | Mucoid phenotypea | Reference or source |
|---|---|---|---|
| P. aeruginosa strains | |||
| PAO1 | Wild type | NM | 38 |
| PAZ-1 | ΔalgZ | NM | This study |
| PAO6886 | algZ::Tcr | NM | 89 |
| PSL317 | ΔalgR | NM | 48 |
| PSL317Z | algZ::Tcr ΔalgR | NM | This study |
| PAO568 | mucA2 | M | 29 |
| PDO300 | mucA22 | M | 54 |
| PAZ568 | mucA2 ΔalgZ | M | This study |
| PAO6884 | mucA2 algZ::Tcr | M | 89 |
| PAR568 | mucA2 ΔalgR | NM | 13 |
| PAO6885 | mucA2 algZ::GmralgR::Tcr | NM | 89 |
| TUMC92 | Clinical isolate | NM | 13 |
| TUMC92Z | ΔalgZ | NM | This study |
| TUMC92R | ΔalgR | NM | 13 |
| FRD-1 | mucA | M | 32 |
| FRD-1Z | mucA ΔalgZ | M | This study |
| FRD-1R | mucA algR::Smr | NM | 50 |
| WFPAPAO1 | Wild type | NM | 50 |
| WFPA8 | algR(D54N) | NM | 50 |
| WFPA13 | algR(D85N) | NM | 50 |
| WFPA16 | algR(D54N D85N) | NM | 50 |
| WFPA8A | mucA algR(D54N) | NM | This study |
| WFPA13A | mucA algR(D85N) | M | This study |
| WFPA16A | mucA algR(D54N D85N) | M | This study |
| WFPA8Z | mucA algR(D54N) | NM | This study |
| WFPA13Z | mucA algR(D85N) | M | This study |
| WFPA16Z | mucA algR(D54N D85N) | M | This study |
| WFPA8AZ | mucA ΔalgZ algR(D54N) | M | This study |
| WFPA13AZ | mucA ΔalgZ algR(D85N) | M | This study |
| WFPA16AZ | mucA ΔalgZ algR(D54N D85N) | M | This study |
| PAO1 vfr | Δvfr | NM | This study |
| PAK | Hyperpiliated | N | 39 |
| PAK mucA | mucA22 | M | 87 |
| PAK algR | algR::Gmr | NM | 87 |
| PAK mucA algR | mucA22 algR::Gmr | NM | 87 |
| PA14 | Wild type | NM | 68 |
| PA103 | Wild type | NM | 47 |
| 19660 | Clinical isolate | NM | 83 |
| X13273 | Clinical isolate | NM | 83 |
| S54485 | Clinical isolate | NM | 83 |
| CF27 | Clinical isolate | M | 83 |
| PAK pscC | ΔpscC | NM | 84 |
| PAK HCN KI | ΔpscC; PAO1 hcnA promoter | NM | This study |
| Plasmids | |||
| pCR2.1 | TA cloning vector; Apr Kanr | Invitrogen | |
| pCVD442 | Gene replacement vector; TcrsacB+ | 26 | |
| pZKO442 | algZ in-frame deletion vector; Apr | This study | |
| pUCP18 | Expression vector; Tcr | 78 | |
| pUCP18Z | algZ expression vector for complementation | This study | |
| pRK2013 | Helper plasmid for conjugation, tra functions | 28 | |
| pEXGmGW | Gateway converted pEX18Gm | 83 | |
| phcnAKI | pEXGmGW with hcnA PAO1 promoter | This study |
a
M, mucoid; NM, nonmucoid.