Effect of glnA disruption on degR-lacZ driven by σD (A)- and σA (B)-dependent RNA polymerase. (A) Open circles, ODM50 (wild type); solid circles, AY50G (glnA); open squares, AY50T (tnrA); solid squares, AY50GT (glnA tnrA). (B) Open circles, ODM612 (wild type); solid circles, AYM612 (glnA). β-Galactosidase activities were determined as described in Materials and Methods. The data set shown is from one of two experiments, and the variations of the enzyme levels between the experiments were within 15%.