TABLE 1.
Bacterial strains and plasmids used in this study
| Species and strain or plasmid | Description | Source or reference |
|---|---|---|
| B. subtilis | ||
| CU741 | trpC2 leuC7 | Laboratory stock |
| AY741G | trpC2 leuC7 glnA::Bsr | 42 |
| AY741T | trpC2 leuC7 tnrA::Cmr | 42 |
| AY741TN | trpC2 leuC7 tnrA::Nmr | pCm::Nm × AY741T |
| TT7151 | trpC2 leuC7 degU::NmraprE::pSKD1 | 20 |
| AY741U | trpC2 leuC7 degU::Nmr | TT7151 × CU741 |
| AY741L | trpC2 leuC7 amyE::lacZ (Cmr) (no promoter) | pIS284 × CU741 |
| OAM145 | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) | 22 |
| AY145G | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) glnA::Bsr | AY741G × OAM145 |
| AY145T | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) tnrA::Nmr | AY741TN × OAM145 |
| AY145U | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) degU::Nmr | AY741U × OAM145 |
| AY145GT | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) glnA::BsrtnrA::Nmr | AY741TN × AY145G |
| OAM147 | trpC2 leuC7 amyE::aprE-lacZ(−299, Cmr) | 22 |
| AY147G | trpC2 leuC7 amyE::aprE-lacZ(−299, Cmr) glnA::Bsr | AY741G × OAM147 |
| AY241 | trpC2 leuC7 amyE::aprE-lacZ(−113, Cmr) | BG4200 × CU741 |
| AY241G | trpC2 leuC7 amyE::aprE-lacZ(−113, Cmr) glnA::Bsr | AY741G × AY241 |
| TSU2 | trpC2 leuC7 amyE::scoC-lacZ(−422, Cmr) | 14 |
| TSU2G | trpC2 leuC7 amyE::scoC-lacZ(−422, Cmr) glnA::Bsr | AY741G × TSU2 |
| TSU2T | trpC2 leuC7 amyE::scoC-lacZ(−422, Cmr) tnrA::Nmr | AY741TN × TSU2 |
| TSU2GT | trpC2 leuC7 amyE::scoC-lacZ(−422, Cmr) glnA::BsrtnrA::Nmr | AY741TN × TSU2G |
| TSU31 | trpC2 leuC7 amyE::scoC-lacZ(−167, Cmr) | pSCO256 × CU741 |
| TSU31G | trpC2 leuC7 amyE::scoC-lacZ(−167, Cmr) glnA::Bsr | AY741G × TSU31 |
| TSU32 | trpC2 leuC7 amyE::scoC-lacZ(−144, Cmr) | pSCO279 × CU741 |
| TSU32G | trpC2 leuC7 amyE::scoC-lacZ(−144, Cmr) glnA::Bsr | AY741G × TSU32 |
| TSU33 | trpC2 leuC7 amyE::scoC-lacZ(−122, Cmr) | pSCO301 × CU741 |
| TSU33G | trpC2 leuC7 amyE::scoC-lacZ(−122, Cmr) glnA::Bsr | AY741G × TSU33 |
| TSU34 | trpC2 leuC7 amyE::scoC-lacZ(−73, Cmr) | pSCO350 × CU741 |
| TSU34G | trpC2 leuC7 amyE::scoC-lacZ(−73, Cmr) glnA::Bsr | AY741G × TSU34 |
| TSU2M | TSU2 carrying sequence alterations at the putative TnrA target site | pSCO256 M × CU741 |
| TSU2MG | Same as TSU2 M except for carrying glnA::Bsr | AY741G × TSU2 M |
| TSU2MT | Same as TSU2 M except for carrying tnrA::Nmr | AY741TN × TSU2 M |
| TSU2MGT | Same as TSU2 M except for carrying glnA::Bsr and tnrA::Nmr | AY741TN × TSU2MG |
| OAM157 | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) scoC::Emr Tcr | 22 |
| AY157G | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) scoC::Emr TcrglnA::Bsr | AY741G × OAM157 |
| AY157U | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) scoC::Emr TcrdegU::Kmr | AY741U × OAM157 |
| AY157GU | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) scoC::Emr TcrglnA::BsrdegU::Kmr | AY741U × AY157G |
| ODM50 | trpC2 leuC7 amyE::degR1-lacZ(−352, Cmr) | 24 |
| AY50G | trpC2 leuC7 amyE::degR1-lacZ(−352, Cmr) glnA::Bsr | AY741G × ODM50 |
| AY50T | trpC2 leuC7 amyE::degR1-lacZ(−352, Cmr) tnrA::Nmr | AY741TN × ODM50 |
| AY50GT | trpC2 leuC7 amyE::degR1-lacZ(−352, Cmr) glnA::BsrtnrA::Nmr | AY741TN × AY50G |
| ODM612 | trpC2 leuC7 sigD amyE::degRm5-lacZ(Cmr) | 24 |
| AYM612G | trpC2 leuC7 sigD amyE::degRm5-lacZ(Cmr) glnA::Bsr | AY741G × ODM612 |
| ODS200 | trpC2 leuC7 sigD-lacZ(Cmr) | 24 |
| AYS200G | trpC2 leuC7 sigD-lacZ(Cmr) glnA::Bsr | AY741G × ODS200 |
| AYS200T | trpC2 leuC7 sigD-lacZ(Cmr) tnrA::Nmr | AY741TN × ODS200 |
| AYS200GT | trpC2 leuC7 sigD-lacZ(Cmr) glnA::BsrtnrA::Nmr | AY741TN × AYS200G |
| ODF200 | trpC2 leuC7 hag-lacZ(Cmr) | 24 |
| AYF200G | trpC2 leuC7 hag-lacZ(Cmr) glnA::Bsr | AY741G × ODF200 |
| BG4136 | trpC2 hisA1 thr-5 degR::Emr | 40 |
| AY145R | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) degR::Emr | BG4136 × OAM145 |
| AY157R | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) scoC::Emr TcrdegR::Emr | OAM157 × AY145R |
| AY157GR | trpC2 leuC7 amyE::aprE-lacZ(−412, Cmr) scoC::Emr TcrdegR::EmrglnA::Bsr | AY741G × AY157R |
| E. coli JM103 | Δ(lac-pro) thi rpsL supE sbcB hsdR4 F′ [traD36 proAB+lacIqlacZΔM15] | 41 |
| Plasmids | ||
| pIS284 | Cmr, E. coli plasmid for insertion of lacZ fusions into B. subtilis amyE locus | I. Smith |
| pCm::Nm | E. coli plasmid to change Cmr to Nmr | M. Steinmetz |
| pSCO256 | pIS284 carrying positions −167 to +139 of the scoC promoter | This study |
| pSCO279 | pIS284 carrying positions −144 to +139 of the scoC promoter | This study |
| pSCO301 | pIS284 carrying positions −122 to +139 of the scoC promoter | This study |
| pSCO350 | pIS284 carrying positions −73 to +139 of the scoC promoter | This study |
| pSCO256 M | pSCO256 carrying altered nucleotides upstream of scoC | This study |