FIG. 4.

EACA blocked plasminogen binding of recombinant enolase of A. hydrophila SSU hyperproduced in E. coli. In a sandwich ELISA, 2 μg of purified recombinant enolase of A. hydrophila was precoated in each well, and plasminogen (2 μg) with or without EACA (2 to 8 mM) was then added to the wells for a 2-h incubation at room temperature. After a washing step, we detected plasminogen binding to the coated enolase by using antiplasminogen antibody. The wells coated with 2 μg of plasminogen were used as positive controls for the system while those coated with PBS only were used as negative controls and set as blanks when the absorbance readings were taken. A total of eight wells were used per group. The asterisk denotes statistically significant differences compared to the group without the addition of EACA (Student's t test).