FIG. 3.

Effects of different arsA gene insertions on alkylresocinol synthesis and on the expression of arsB. (A) Alkylresorcinol staining of SW136 and different asrA mutants. The cells were induced to encyst on Burk-butanol medium for 5 days. (B) Effects of different arsA insertions on the expression of arsB, measured by real-time reverse transcription-PCR. The levels of the arsB transcripts were measured under encystment-inducing conditions and were normalized according to the level of the gyrA mRNA. The data are presented as fold changes of mRNA levels of OV11, SW-A, and SW-AP mutant strains relative to those of the parental strain (SW136). These data represent the means of triplicates, and the error bars represent the standard deviations.