TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics | Source or reference(s) |
|---|---|---|
| A. vinelandii strains | ||
| OP | Natural algU mutant of strain O, nonmucoid | 4, 15 |
| UW136 | Natural rifampin-resistant mutant of OP, nonmucoid | 3 |
| SW136 | algU+ derivative of UW136, mucoid | This work |
| OV8 | SW136 with an arsA::Tn5SSgusA40 insertion, mucoid | This work |
| OV11 | SW136 with an arsA::Tn5SSgusA40 insertion, mucoid | This work |
| SW-A | SW136 with an arsA::Gmr insertion, mucoid | This work |
| SW-AP | SW136 with an arsA::Gmr insertion opposite to arsA, mucoid | This work |
| SW-B | SW136 with an arsB::Gmr insertion, mucoid | This work |
| E. coli strains | ||
| DH5α | supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 9 |
| S17-1 λpir | thi pro hsdR hsdM+recA RP4 2-Tc::Mu-Km::Tn7(Tpr/Smr) λ-pir | 27 |
| Plasmids | ||
| pCAM140 | Vector containing the mTn5SSgusA40 for mutagenesis, (promoterless gusA for transcriptional fusions) | 27 |
| pBluescript KS(+) | Plasmid for subcloning DNA | Stratagene |
| pMOSBlue | Plasmid for cloning PCR products | Amersham |
| pBSL141 | Source of gentamicin resistance cassette | 1, 6 |
| pSMU85 | pBluescript KS(+) carrying algU from A. vinelandii ATCC 9046 | 18 |
| pOV8 | pBluescript KS(+) carrying 7-kb PstI fragment containing Tn5SSgusA40 insertion of OV8 | This work |
| pOV11 | pBluescript KS(+) carrying 7-kb PstI fragment containing Tn5gusA40 insertion of OV11 | This work |
| pSMarsA-Gm | pMOSBlue derivative carrying an arsA::Gmr insertion | This work |
| pSMarsA-GmP | pMOSBlue derivative carrying an arsA::Gmr insertion opposite to arsA | This work |
| pYRC1n | pMOSBlue derivative carrying an arsB::Gmr insertion | This work |