FIG. 3.

The effect of E. coli rpoS on mazEF-mediated cell death during stationary phase under anaerobic conditions. Strains described in the legend of Fig. 1 were grown under anaerobic conditions until the stationary phase (OD₆₀₀ = 1.3 to 1.4). Stressful conditions were induced by incubation of cells at 37°C, without shaking them, with rifampin (10 μg/ml) for 10 min (A); spectinomycin (1 mg/ml) for 10 min (B); or trimethoprim (5 μg/ml) for 1 h (C). For the rest of the experimental details, see the text.