Sequence alignment of the goldfish GnRH receptor subtypes A and B to the human and catfish GnRH receptors. The putative transmembrane (TM) domains are indicated. cf, catfish; GfB, goldfish B; GfA, goldfish A; h, human. The two goldfish receptor subtypes A and B share 71% amino acid identity. The GfB GnRH receptor has an 82% amino acid identity to the catfish GnRH receptor, and the GfA subtype shows a lower identity of 71% to this receptor. All the fish receptors have amino acid identities of between 42% and 43% to the human GnRH receptor. Upper numbering refers to GfB, while lower numbering refers to GfA.