TABLE 2.

Primers, T_anns used for amplification, and positions in the amplicons used for assigning allelic types

Primer	Nucleotide sequence (5′→3′)	Tann (°C)a	Amplicon size (bp)	Position in the amplicon used for MLST (bp)
atpD forw	ATGAGTCAGGGCAAGATCGTTC	62	858	214-744
atpD rev	TCCTGCAGGACGCCCATTTC
gapA forw	TGGCAATCAAGGTTGGTATCAAC	62	800	120-677
gapA rev	TTCGCTCTGTGCCTTCACTTC
guaA forw	AACGAAGAAAAGCGCTGGTA	(62)	704	70-621
guaA rev	ACGGATGGCGGTAGACCAT
mutM forw	AACTGCCCGAAGTCGAAAC		579	42-506
mutM rev (2r)	GAGGATCTCCTTCACCGCATC	58 (62)
mutM rev (4r)	TTACCGGCCTCGCGCAG	52 (48)	545	42-506
nuoD forw	TTCGCAACTACACCATGAAC	48	514	33-476
nuoD rev	CAGCGCGACTCCTTGTACTT
ppsA forw	CAAGGCGATCCGCATGGTGTATTC	62	635	65-559
ppsA rev	CCTTCGTAGATGAA(A/G)CCGGT(A/G)TC
recA forw	ATGGACGAGAACAAGAAGCGC	62	807	100-645
recA rev	CCTGCAGGCCCATCGCC

^aThe value in parentheses represents the T_ann in the presence of 1.3 M betaine.