TABLE 2.
Bioinformatics analysis of nonannotated ORFs in the H. volcanii agl cluster
| ORF | Homologue determined by:
|
BLAT result | Predicted protein
|
||
|---|---|---|---|---|---|
| Conserved-domain BLAST | BLAST | No. of amino acids | pI | ||
| HVO_1518 | None | Transposase (ISH5) | 15 | 95 | 5.2 |
| HVO_1519 | Transposase | Transposase (ISH3) | 39 | 397 | 6.0 |
| HVO_1520 | Transposase | Transposase (ISH5) | 14 | 295 | 7.1 |
| HVO_1521 | None | Hypothetical | 14 | 43 | 4.6 |
| HVO_1522a | None | Methyltransferase | 0 | 239 | 4.8 |
| HVO_1523 | None | Hypothetical | 0 | 469 | 5.5 |
| HVO_1524 | Involved in O-antigen export | Polysaccharide biosynthesis/transport | 0 | 476 | 9.0 |
| HVO_1525 | Transposase | Transposase (ISH3) | 37 | 396 | 5.6 |
| HVO_1526 | None | Hypothetical | 0 | 457 | 6.1 |
| ORF 2 | None | None | 38 | 136 | 5.5 |
| ORF 3 | Transposase | Transposase | 38 | 177 | 10.2 |
| ORF 5 | None | Hypothetical | 0 | 371 | 5.6 |
| ORF 7 | Involved in O-antigen export | Polysaccharide biosynthesis/transport | 0 | 493 | 8.7 |
| ORF 8 | None | Transposase | 37 | 177 | 9.9 |
| ORF 9 | None | Hypothetical | 37 | 149 | 12.1 |
| ORF 10 | Transposase | None | 0 | 457 | 6.1 |
a
HVO_1522 = ORF 4.