TABLE 1.
Bacterial strains and vectors used in this study
| Strain or plasmid | Characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| P. syringae | ||
| DC3000 | Derivative of NCPPB1106; Rifr | 8 |
| AK4C9 | mqo::Tn5 uidA; Rifr Kmr | This study |
| mqo::KO | mqo gene replaced with Sm/Specr gene; Rifr Specr | This study |
| DC3000 pMQO | DC3000 with pMQO clone; Rifr Tetr | This study |
| DC3000 pME6031 | DC3000 with pME6031; Rifr Tetr | This study |
| mqo::KO pMQO | mqo::KO with pMQO clone; Rifr Specr Tetr | This study |
| mqo::KO pME6031 | mqo::KO with pME6031; Rifr Specr Tetr | This study |
| dctA1::KO | dctA1 gene replaced with Sm/Specr gene; Rifr Specr | This study |
| dctA2::pJP5603 | dctA2::disrupted with pJP5603; Rifr, Kmr | This study |
| dctA1::KO dctA2::pJP5603 | dctA1::KO; dctA2::pJP5603; Rifr Specr Kmr | This study |
| E. coli | ||
| DH5α λpir | recA, lacZΔM15, λpir | 35 |
| MM294A (pRK600) | Triparental mating helper strain; Cmr | 12 |
| Plasmids | ||
| pHP45Ω | Source of Ω cassette; Sm/Specr gene | 37a |
| pRK415 | Broad-host-range vector; Tetr | 21a |
| pJP5603 | Suicide vector; Kmr | 36a |
| pmqo-pRK415 | Construct used to make mqo::KO; Tetr | This study |
| pmqo::Spec-pRK415 | Construct used to make mqo::KO; Specr Tetr | This study |
| pmqo::KO | Construct used to make mqo::KO strain; Specr Kmr | This study |
| pdctA1up-pJP5603 | Construct used to make dctA1::KO plasmid; Tetr | This study |
| pdctA1down-TOPO | Construct used to make dctA1::KO plasmid; Kmr | This study |
| pdctA1::KO | Construct used to make dctA1::KO strain; Specr Tetr | This study |
| pdctA2::pJP5603 | Construct used to make dctA2::pJP5603 strain; Kmr | This study |
| pCR-Blunt II TOPO | Cloning vector; Kmr | Invitrogen |
| pME6031 | Replicating vector, stable in P. syringae; Tetr | 18 |
| pMQO | Wild-type mqo gene in pME6031; Tetr | This study |
| pDctA1 | Wild-type dctA1 gene in pME6031; Tetr | This study |
a
Cmr, chloramphenicol resistance; Tcr, tetracycline resistance; Rifr, rifampin resistance; Specr, spectinomycin resistance; Kmr, kanamycin resistance.