TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Relevant characteristics | Source or reference |
|---|---|---|---|
| Strains | |||
| jf570 | H10407ΔeltA | ETEC strain with polar insertion in eltA (LT deficient) | 9 |
| MK1052 | H10407ΔeltA/pILT | jf570 carrying inducible LT plasmid; Ampr | This work |
| MK1200 | H10407ΔeltA/pILT[T47A] | jf570 carrying inducible T47A mutant; Ampr | This work |
| MK1201 | H10407ΔeltA/pILT[Q3K] | As described for MK1200, with Q3K; Ampr | This work |
| MK1203 | H10407ΔeltA/pILT[Q3L] | As described for MK1200, with Q3L; Ampr | This work |
| MK1204 | H10407ΔeltA/pILT[Y18A] | As described for MK1200, with Y18A; Ampr | This work |
| MK1206 | H10407ΔeltA/pILT[A46D] | As described for MK1200, with A46D; Ampr | This work |
| MK741 | DH5α degP::Tn5/pDsbA/pILT | E. coli K-12, degP knockout, carrying a plasmid copy of dsbA and an inducible LT plasmid; Kanr Cmr Ampr | 25 |
| MK1207 | DH5α degP::Tn5/pDsbA/pILT[T47A] | As described for MK741, with inducible T47A; Kanr Cmr Ampr | This work |
| MK1208 | DH5α degP::Tn5/pDsbA/pILT[Q3K] | As described for MK1207, with Q3K; Kanr Cmr Ampr | This work |
| MK1210 | DH5α degP::Tn5/pDsbA/pILT[Q3L] | As described for MK1207, with Q3L; Kanr Cmr Ampr | This work |
| MK1211 | DH5α degP::Tn5/pDsbA/pILT[Y18A] | As described for MK1207, with Y18A; Kanr Cmr Ampr | This work |
| MK1213 | DH5α degP::Tn5/pDsbA/pILT[A46D] | As described for MK1207, with A46D; Kanr Cmr Ampr | This work |
| CWG311 | F470 waaV::aacC1 | R1 core, lacks O antigen; Gmr | 15 |
| CWG309 | F470 waaT::aacC1 | R1 core, two terminal Gal residues removed; Gmr | 15 |
| CWG303 | F470 waaG::aacC1 | R1 core, entire outer core removed; Gmr | 15 |
| WBB01 | JC7236 ΔwaaCF::tet6 | Expresses only Kdo2-lipid A; Tetr | 3 |
| Plasmids | |||
| pILT | IPTG-inducible LT holotoxin; Ampr | This work | |
| pDsbA | dsbA ligated into pACYC184; Cmr Tetr | This work |