FIG. 1.

Distribution of GFP chimeras varies with expression level and context. Plasmid-borne GFP-EpsM in live cells of V. cholerae epsM mutant PU3 was polarly localized when overexpressed with 10 μM IPTG (A) but circumferentially distributed when not induced (B). Both patterns differed from that of chromosomally expressed GFP-EpsM, balanced with the other Eps proteins, which formed fluorescent foci along the cell membranes (C). (D) GFP-EpsC, expressed from the chromosome, similarly displayed fluorescent foci along the full lengths of the cells. (E and F) Both GFP-EpsM and GFP-EpsC fluorescent foci dissipated upon coexpression of IPTG-induced, plasmid-encoded native EpsM and EpsC, respectively.