TABLE 1.
Strains and plasmids
| Strain, genotype, or plasmid | Features | Reference or source |
|---|---|---|
| V. cholerae strains or genotypes | ||
| TRH7000 | El Tor strain, wild type for T2S | 23 |
| PBAD::eps | TRH7000 PBAD::eps | 55 |
| ΔepsC | In-frame replacement of epsC with aph-3 (Kmr) | This study |
| ΔepsD | In-frame replacement of epsD with aph-3 (Kmr) | This study |
| ΔepsG | In-frame replacement of epsG with cat (Cmr) | This study |
| ΔepsL | In frame replacement of epsL with aph-3 (Kmr) | 55 |
| PU3 (epsM mutant) | Tn5 insertion in epsM at position 247 (Kmr) | 39 |
| gfp-epsC | TRH7000 with gfp-epsC replacement at epsC locus | This study |
| gfp-epsC ΔepsD | gfp-epsC with replacement of epsD with aph-3 (Kmr) | This study |
| gfp-epsC ΔepsL | gfp-epsC with replacement of epsL with aph-3 (Kmr) | This study |
| gfp-epsC epsM mutant | PU3 with replacement of epsC with gfp-epsC | This study |
| gfp-epsM | TRH7000 with gfp-epsM replacement at epsM locus | This study |
| gfp-epsM ΔepsC | gfp-epsM with replacement of epsC with aph-3 (Kmr) | This study |
| gfp-epsM ΔepsD | gfp-epsM with replacement of epsD with aph-3 (Kmr) | This study |
| gfp-epsM ΔepsL | gfp-epsM with replacement of epsL with aph-3 (Kmr) | This study |
| PBAD::eps gfp-epsC | TRH7000 PBAD::eps with gfp-epsC replacement at epsC locus | This study |
| PBAD::eps gfp-epsM | TRH7000 PBAD::eps with gfp-epsM replacement at epsM locus | This study |
| PBAD::eps gfp-epsC ΔepsD | PBAD::eps gfp-epsC with replacement of epsD with aph-3 (Kmr) | This study |
| PBAD::eps mcherry-epsC gfp-epsM | TRH7000 PBAD::eps with mcherry-epsC replacement at epsC locus and gfp-epsM replacement at epsM locus | This study |
| E. coli strains | ||
| MC1061 | F−lac mutant; K-12 laboratory strain | 9 |
| MM294/pRK2013 | Helper strain for conjugations | 33 |
| SY327 λpir | λpir lysogen; permits replication of pCVD442 | 35 |
| Plasmids | ||
| pMMB66 | Low-copy, IPTG-inducible vector (Apr) | 19 |
| pMMB67 | Low-copy, IPTG-inducible vector (Apr) | 19 |
| pCVD442 | Suicide vector containing sacB (Apr) | 14 |
| pUC18K | aphA-3 gene (Kmr, Apr) | 32 |
| pMMB68 | etxB (heat-labile enterotoxin B subunit gene) (Apr) | 47 |
| pBAD33 | cat gene (Cmr) | 22 |
| pK18mobsacB | Suicide vector containing sacB (Kmr) | 51 |
| pEpsC | pMMB67EH-epsC | This study |
| pEpsCD | pMMB67-epsC-epsD | This study |
| pEpsD | pMMB67EH-epsD | This study |
| pEpsG | pMMB67EH-epsG | This study |
| pMS44 (pEpsL) | pMMB67HE-epsL | 47 |
| pMMB524 (pEpsM) | pMMB67EH-epsM | 39 |
| pGFP-EpsM | pMMB-gfp-epsM | 53 |
| pmCherry-EpsCD | pMMB67EH-mcherry-epsCD | This study |
| pVC1200 | pMMB66-vc1200 | This study |