TABLE 2.
Primers used for plasmid constructions
| Primer | Sequence (5′-3′) | Feature(s) | Construct(s) generated |
|---|---|---|---|
| epsC01 | CGGATCCGAATTTAAACAACTTCCTC | BamHI | pGFP-EpsC, pGFP-EpsCD |
| epsC02 | TCTGCAGGTCGTTACTCGCCTTAG | PstI | pGFP-EpsC |
| epsC03 | CTCTAGATGGCAATATGAAGCGTA | XbaI | pBAD33-EpsC |
| epsD01 | ATCTAGATCGGTCATTGCTTGGCTTC | XbaI | pGFP-EpsCD |
| epsD03 | GGATCCGAGTAACGACGCCTTA | BamHI | pEpsD |
| epsD04 | GCATGCGCTGGAGAGATCACCA | SphI | pEpsD |
| epsG-OEup | GGCGAATTCGACATAGTGTGGA | EcoRI | pEpsG |
| epsG-OEdn | TTCCGTCGACTACCGCTAATTAG | SalI | pEpsG |
| epsC-ko1 | CCGAGCTCACCTTGATAGCGC | SacI | ΔepsC vector |
| epsC-ko2 | CGGAGGTACCTGTTTAAATTCCATA | KpnI | ΔepsC vector |
| epsC-ko3 | GATGGATCCCAACATGATGTAT | BamHI | ΔepsC vector |
| epsC-ko4 | CTCTAGATTACGTTTTGAAGTG | XbaI | ΔepsC vector |
| epsD-ko1 | CTGAGCTCGTGTTATATTGCGATG | SacI | ΔepsD vector |
| epsD-ko2 | CTAAGGTACCGTTACTCGCCTTA | KpnI | ΔepsD vector |
| epsD-ko3 | CTTTATGGATCCGATGGAAGCCAAG | BamHI | ΔepsD vector |
| epsD-ko4 | CCGTCTAGAAATCACGAATTTCC | XbaI | ΔepsD vector |
| epsD-ko1gfpC | CTTTATTGTCTAGATGGAAGCCAAG | XbaI | gfp-epsC ΔepsD vector |
| epsD-ko2gfpC | GCCGCATGCAAATCACGAATTTC | SphI | gfp-epsC ΔepsD vector |
| gfp03 | CCTGAGCTCTTACCAGACAACCA | SacI | gfp-epsC ΔepsD vector (in gfp) |
| CmR-1 | GTTGTCGACATTTTCAGGAGCTAA | SalI | ΔepsG vector |
| CmR-2 | AGCTGCAGGCGTTTAAGGGCA | PstI | ΔepsG vector |
| epsG-ko1 | CAGTCTAGAAAACCGCGGATTCG | XbaI | ΔepsG vector |
| epsG-ko2 | GGGTCGACCCCGTTTGTTTAC | SalI | ΔepsG vector |
| epsG-ko3 | GGTAACTGCAGTATCCAAGATTTTC | PstI | ΔepsG vector |
| epsG-ko4 | TACGCATGCTCACGACTGGG | SphI | ΔepsG vector |
| epsL-ko1 | GAGCTCTTAATTGTGATTCTGCTCCT | SacI | ΔepsL vectors |
| epsL-ko2 | GGTACCAAACCAGCCAAGGGATATC | KpnI | ΔepsL vectors |
| epsL-ko3 | TCTAGAGTTTGTGGTGAAGCCCAAG | XbaI | ΔepsL, ΔepsL-gfpM vectors |
| epsL-ko4 | GTCGACAAGCTAAGCTGCCTTCG | SalI | ΔepsL vector |
| epsL-ko5gfpM | GTCGACTTGAACTGACGCGTCGA | SalI | ΔepsL-gfpM vector |
| gfpCchr1 | AGTTCTTCTCCTTTACTCATAAATTTCCACGTTATTCCTT | epsC rbs and gfp junction | Vector for creation of gfp-epsC strain |
| gfpCchr2 | AAGGAATAACGTGGAAATTTATGAGTAAAGGAGAAGAACT | epsC rbs and gfp junction | Vector for creation of gfp-epsC strain |
| gfpCchr3 | GGCCCGGGACTCGTTTGCCAT | SmaI | Vector for creation of gfp-epsC strain |
| epsL21 | GTCAGCATGCAAATATGCTGCC | SphI | Vector for creation of gfp-epsM strain |
| epsM28 | ATGCAGGTCTGGATCCAACC | BamHI (natural) | Vector for creation of gfp-epsM strain |
| epsN03 | CCAAGCTGCAGCGCACCAAT | PstI (natural) | Vector for creation of gfp-epsM strain |
| gfpMchr1 | AGTTCTTCTCCTTTACTCATTTCTCCTTACTTGGGCTTCA | epsM rbs and gfp junction | Vector for creation of gfp-epsM strain |
| gfpMchr2 | TGAAGCCCAAGTAAGGAGAAATGAGTAAAGGAGAAGAACT | epsM rbs and gfp junction | Vector for creation of gfp-epsM strain |
| epsN04 | CCAAGCCCGGGCGCACCAAT | SmaI | Vector for creation of gfp-epsM strain |
| mcherry-up | GGTACCATGGTAAGCAAGGGCGA | KpnI | pmCherry |
| mcherry-dn | GGATCCCTTGTACAGCTCGTCCAT | BamHI | pmCherry |
| PBAD-mcherry overlap | AAGGAATAACGTGGAAATTTATGGTAAGCAAGGGCGAGGA | PBAD promoter region and mCherry junction | Vector for creation of PBAD::eps mcherry-epsC gfp-epsM |
| PBAD-mg-up | TCTAGAACTGCTGGCGGAAAAGATGTGAC | XbaI | Vector for creation of PBAD::eps gfp-epsC and PBAD::eps gfp-epsM |
| EpsC-mg-down | GCATGCAAACTGCCCGCCAACAGCCAT | SphI | Vector for creation of PBAD::eps gfp-epsC |
| PBAD::gfpepsC overlap | GAATAACGTGGAAATTTATGAGTAAAGGAGAAG | PBAD promoter region and gfp junction | Vector for creation of PBAD::eps gfp-epsC |
| VC1200-f | GAATTCAGGATTGAGTTGTGCATCAG | EcoRI | pVC1200 |
| VC1200-r | CTGCAGATCTCGGATAGGTAATCAAG | PstI | pVC1200 |