TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description and/or relevant characteristic(s)a | Expressionb of:
|
Reference or sourcec | |
|---|---|---|---|---|
| Gelatinase | Bacteriocin MC4-1 | |||
| E. faecalis strains | ||||
| MC4 | Root canal isolate; contains pAMS1 | + | − | 9 |
| FA2-2 | Rfr Far | − | NA | 4 |
| SFF2 | FA2-2/pAMS1 | − | + | 11 |
| JH2-2 | Rfr Far | − | NA | 15 |
| SFJ1 | JH2-2/pAMS1 | − | + | 11 |
| SF411 | JH2-2/pAMS411 | − | + | 11 |
| SF412 | JH2-2/pAMS412 | − | + | 11 |
| JH2SS | Smr Spr | − | NA | 27 |
| SFJS1 | JH2SS/pAMS1 | − | + | 11 |
| OG1RF | Rfr Far | + | NA | 7 |
| SFOR2 | OG1RF/pAMS1 | + | − | 11 |
| OG1SS | Smr Spr | + | NA | 12 |
| SFOS2 | OG1SS/pAMS1 | + | − | 11 |
| TX5128 | Gel− derivative of OG1RF; Rfr Far | − | NA | 26 |
| SFTX2 | TX5128/pAMS1 from SFJS1 × TX5128 filter mating | − | + | This study |
| SFTX3 | TX5128/pAMS1 from SFOS2 × TX5128 filter mating | − | + | This study |
| GS18 | Root canal isolate | − | NA | 25 |
| GS18e | Emr | − | NA | This study |
| GS18e/pAMS1 | − | + | This study | |
| GS25 | Root canal isolate | − | NA | 25 |
| GS25e | Emr | − | NA | This study |
| GS25e/pAMS1 | − | + | This study | |
| E5 | Oral isolate | − | NA | 24 |
| E5e | Emr | − | NA | This study |
| E5e/pAMS1 | − | + | This study | |
| AA-OR4 | Oral isolate | − | NA | 23 |
| AA-OR4e | Emr | − | NA | This study |
| AA-OR4e/pAMS1 | − | + | This study | |
| GS3 | Root canal isolate | + | NA | 25 |
| GS3e | Emr | + | NA | This study |
| GS3e/pAMS1 | + | − | This study | |
| GS6 | Root canal isolate | + | NA | 25 |
| GS6e | Emr | + | NA | This study |
| GS6e/pAMS1 | + | − | This study | |
| GS16 | Root canal isolate | + | NA | 25 |
| GS16e | Emr | + | NA | This study |
| GS16e/pAMS1 | + | − | This study | |
| E6 | Oral isolate | + | NA | 24 |
| E6e | Emr | + | NA | This study |
| E6e/pAMS1 | + | − | This study | |
| ER5/1 | Root canal isolate | + | NA | 16 |
| ER5/1e | Emr | + | NA | This study |
| ER5/1e/pAMS1 | + | − | This study | |
| Enterococcus faecium 409 | NA | NA | UMH | |
| Listeria monocytogenes ATCC 19115 | NA | NA | ATCC | |
| Plasmids | ||||
| pAMS1 | Cmr Smr Tcr; encodes bacteriocin MC4-1 and response to pheromone cCF10 | 11 | ||
| pAMS411 | Emr; PCR-generated bacAB clone; orientation in vector pAMS749-12 opposite to pAMS412 | 11 | ||
| pAMS412 | Emr; PCR-generated bacAB clone; orientation in vector pAMS749-12 opposite to pAMS411 | 11 | ||
| pVA749 | Emr | 17 | ||
a
Gel, gelatinase; Cm, chloramphenicol; Em, erythromycin; Fa, fusidic acid; Rf, rifampin; Sm, streptomycin; Sp, spectinomycin; Tc, tetracycline.
b
When used as a producer with indicators E. faecium 409 and L. monocytogenes ATCC 19115, as described previously (11). +, positive for expression; −, negative for expression; NA, not applicable.
c
ATCC, American Type Culture Collection; UMH, University of Michigan Hospital.