TABLE 2.
Spectrum of activity of MC4-1a bacteriocin from gelatinase-negative and gelatinase-positive strains
| Indicator strain | Bacteriocin activity from gelatinase-negative strain:
|
Bacteriocin activity from gelatinase-positive strain:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| SFJ1 | SFJS1 | SFF2 | SFTX3b | E5e/pAMS1 | AA-OR4e/pAMS1 | GS18e/pAMS1 | GS25e/pAMS1 | MC4c | SFOR2b | SFOS2b | |
| Gelatinase-negative strains | |||||||||||
| SFJ1 | − | − | − | (+) | − | − | − | − | (+) | (+) | (+) |
| SFJS1 | + | − | (+) | (+) | − | − | − | − | (+) | (+) | (+) |
| SFF2 | − | − | − | − | − | − | − | − | (+) | (+) | (+) |
| SFTX3 | + | + | + | + | + | + | + | + | (+) | − | − |
| E5e/pAMS1 | + | + | + | + | + | + | + | + | (+) | − | − |
| AA-OR4e/pAMS1 | + | + | + | + | + | + | + | + | (+) | − | − |
| GS18e/pAMS1 | + | + | + | + | + | + | + | + | (+) | (+) | (+) |
| GS25e/pAMS1 | + | + | + | + | + | + | + | + | (+) | − | − |
| Gelatinase-positive strains | |||||||||||
| MC4 | + | + | + | + | + | + | + | + | − | − | (+) |
| SFOR2 | + | + | + | + | + | + | + | + | (+) | − | − |
| SFOS2 | + | + | + | + | + | + | + | + | (+) | − | − |
a
Activity was evaluated by agar diffusion assays as described previously (11). −, no zone of inhibition (lawn grows up to, and under, producer); +, zone of inhibition; (+), very small zone of inhibition.
b
Bacteriocin MC4-1 from SFOR2 and SFOS2 is not detected (and immunity to MC4-1 activity is not shown). However, an unrelated activity that is characteristic of the OG1 family is expressed by SFTX3, SFOR2, and SFOS2 (11).
c
Bacteriocin MC4-1 from pAMS1 in MC4 is not detected (and immunity to MC4-1 activity is not shown); however, at least one additional bacteriocin activity is expressed (11).