TABLE 2.
Plasmids used during this study
| Plasmid | Relevant characteristics | Source or referencea |
|---|---|---|
| pKD46 | bla PBAD gam beto exo pSC101 oriTS | 15 |
| pCP20 | bla cat λcI857 λPRflp pSC101 oriTS | 10 |
| pKD3 | bla FRT cm FRT oriR6K | 15 |
| pKD4 | bla FRT kan FRT oriR6K | 15 |
| plnt-ts | bla lnt oriR6K | 29 |
| pQE80L | bla lacIq ColE1 | Qiagen |
| pAH125 | kan lacZ attλ oriR6K | 29 |
| pVenus | kan venus attλ oriR6K | |
| PfimA-Venus | kan PfimA venus attλ oriR6K | |
| PfimY-Venus | kan PfimY venus attλ oriR6K | |
| PfimZ-Venus | kan PfimZ venus attλ oriR6K | |
| Pfimw-Venus | kan Pfimw venus attλ oriR6K | |
| PfimU-Venus | kan PfimU venus attλ oriR6K | |
| PhilE-Venus | kan PhilE venus attλ oriR6K | |
| pPROTet.E | cm PLtetO-1ori ColE1 | Stratagene |
| pSS012 (pPROTet.E tetR) | cm PLtetO-1tetR ori ColE1 | 45 |
| pSS039 (pFimY) | cm PLtetO-1fimY tetR ori ColE1 | |
| pSS040 (pFimZ) | cm PLtetO-1fimZ tetR ori ColE1 | |
| pSS041 (pFimW) | cm PLtetO-1fimW tetR ori ColE1 | |
| pSS042 (pFimY*) | cm PLtetO-1fimY* tetR ori ColE1 |
a
Plasmids are from this study unless specified otherwise.