TABLE 3.
List of primers used in the study
| Primer | Sequence | Characteristic |
|---|---|---|
| SS105F | TGT CCG TTA TTG TGG CTC CCG AAC GAT AAT TCG CCG GGA GGA TGG TAG TGT GGG GTC TCC | fimYZ knockout primer |
| SS105R | ATC AAT CAG TTT CTT TAA TAT TTC ACC ATG ATT CAC CTG CCA TGG GAA TTA GCC ATG GTC C | fimYZ knockout primer |
| SS105FII | TTA TAA AAC GAA GGA CGC ATA ACA GTC TGA GGC ATA CAA ATG GGA ATT AGC CAT GGT CC | fimZ knockout primer |
| SS105RII | GTA ATT TCT TAA AAA ATC TTA TTC ACC AAA ACG TTA CTT CGA TGG TAG TGT GGG GTC TCC | fimY knockout primer |
| SS152F | TAT TTC ACC ATG ATT CAC CTG CCG TGT AGG ATA TTT TTT TGT GTA GGC TGG AGC TGC TTC | fimW knockout primer |
| SS152R | GGT GAG ATA TTT CGT AAG CCT TGT AAA AAG TTA AGT GAG TCA TAT GAA TAT CCT CCT TAG | fimW knockout primer |
| SS165F | CTC GCG TTT CGT CTA CAC GAA GTC TTC ACT TCA CAA GGC GGT GTA GGC TGG AGC TGC TTC | fimU knockout primer |
| SS165R | GGA AAA TAA GGA GGA AAT AAA GAA GCG TAA CAC GTT GAT TCA TAT GAA TAT CCT CCT TAG | fimU knockout primer |
| LC294F | GAT TAA CTT TAT AAG GAG GAA AAA CAT ATG AGT AAA GGA GAA GAA CTT TTC | venus gene |
| LC296R | ATA AAG CTT TTA TTT GTA TAG TTC ATC CAT GCC ATG | venus gene |
| LC295F | CTC GAA TTC CCT AAC TAA CTA AAG ATT AAC TTT ATA AGG AGG A | venus gene |
| SS104F | TTT GGT ACC AAA TCT GTG AGG CCG GAT TG | fimA promoter |
| SS104R | GGG GAA TTC GTA GAG GTC ATT AAT TTA TG | fimA promoter |
| SS037F | TCT GGT ACC AAA ATA TAT TAG AGT TAA CC | fimY promoter |
| SS037R | AAA GAA TTC CCC TGC GTG GTA CGC TGC GC | fimY promoter |
| SS103F | TTT GGT ACC ATA AAA CCT CCG CTA TAA CA | fimZ promoter |
| SS103R | GGG GAA TTC CCA TAA TGA TAA CAG ATG CA | fimZ promoter |
| SS154F | GGG GGT ACC GGA TTC GAA CCT GCG ACC CA | fimW promoter |
| SS154R | GGG GAA TTC TTT TCC GGG TAA TTT CTT CA | fimW promoter |
| SS162F | GGG GGT ACC CGT TTC GCT TAA ATG ATA AC | fimU promoter |
| SS162R | GGG GAA TTC CTA TCC AAC TGA GCT AAG GG | fimU promoter |
| SS024F | AAG GGT ACC ATG ACG TTG CGT AGC GTT GG | hilE promoter |
| SS024R | GGT GAA TTC GAA AGA ACG TTC CAT TTT CC | hilE promoter |
| SS106F | GGG GAA TTC TAA CAG TCT GAG GCA TAC AA | fimZ gene |
| SS106R | TTT GGT ACC TTA CAA TAA TTC GTG TGA TT | fimZ gene |
| SS107F | CCT GTC GAC ATA TTA GAG CAA TGG AAA A | fimY gene |
| SS107R | CCC GGA TCC TTA AAA AAT GTC GTG GAA AG | fimY gene |
| SS160F | ATA GAA TTC GCC GTG TAG GAT ATT TTT TT | fimW gene |
| SS160R | ATA AAG CTT TTA TTA CTT ACT GAG TAA GAA ATG AAG G | fimW gene |
| SS162F | GGG GGT ACC ATG CGC AGC GTA CCA CGC CGG GAA CGA CAC CGC CGT TTA CGA AAT GCT AA | fimY* gene |
| SS167F | GGG GAA TTC TTT ATA AGG AGG AAA AAC ATA TGC GCA GCG TAC CAC GCC GG | fimY* gene |