TABLE 3.
Comparison of multiplex, real-time PCR results with AccuProbe or HPLC results for identification of 314 clinical isolates of mycobacteria
| PCR result | No. of isolates with the following result:
|
|||||
|---|---|---|---|---|---|---|
| MTC by probe | MAC by probe | MCAG by HPLC | MFG by HPLC | M. mucogenicum by HPLC | Other AFB by HPLC | |
| MTC | 96 | 0 | 0 | 0 | 0 | 0 |
| MAC | 0 | 96 | 0 | 0 | 0 | 0 |
| MCAG | 0 | 0 | 68 | 0 | 0 | 0 |
| MFG | 0 | 0 | 0 | 18 | 0 | 0 |
| M. mucogenicum | 0 | 0 | 0 | 1a | 9 | 0 |
| Other AFBb | 0 | 1c | 0 | 0 | 0 | 22 |
| Indeterminated | 0 | 1 | 0 | 0 | 1 | 1 |
a
Identified as M. alvei by 16S rRNA sequencing.
b
AFB for which PCR amplified only the mycobacterial genus target.
c
Identified as M. paraffinicum by 16S rRNA sequencing.
d
The eight genus targets that did not interfere with isolate identification are not shown.