TABLE 2.
Oligonucleotide probes used in this study
| Species | Probe | Sequence (5′-3′)a | Probe length (mer) | Tm (°C) | Locationb | GenBank accession no. |
|---|---|---|---|---|---|---|
| L. anisa | Lagtwc | CCTTCAGACTTAGCTTAT(T) | 18 | 35.1 | 1072-1089 | U91607 |
| Lan | GGCGTAACGGTACTGCC(T) | 17 | 48.5 | 832-848 | U91607 | |
| L. birminghamensis | Lbi1 | CAGCGGACTCCAGTACA(T) | 17 | 43.6 | 412-428 | U91608 |
| Lbi2 | AACTGGCAGCGGTGCAA(T) | 17 | 53.1 | 439-455 | U91608 | |
| L. bozemanii | Lbo | (T)CCTTCAGATTTAGCGTA | 18 | 39.6 | 1070-1087 | U91609 |
| L. cincinnatiensis | Lci | AGGTAAGTCAGACACTGTAA(T) | 20 | 37.9 | 855-874 | U91636 |
| L. dumoffii | Ldu1 | AAGAGAATAAAGCAAAAGGC(T) | 20 | 45.5 | 747-766 | U91637 |
| Ldu2 | CAGGTTCAGGCGCTAAGCC(T) | 19 | 54.9 | 846-864 | U91637 | |
| L. feeleii | Lfe1d | AAAATAATCCAGGCTGCTA(T) | 19 | 44.1 | 778-796 | U92205 |
| Lfe2 | AAGCGTTCATGAGCCAGA(T) | 18 | 48.6 | 713-730 | U92205 | |
| L. gormanii | Lagtwc | CCTTCAGACTTAGCTTAT(T) | 18 | 35.1 | 1072-1089 | U91607 |
| Lgo1 | CAGCGCAGAGTTTAACAAGA(T) | 20 | 48.4 | 613-632 | U91638 | |
| Lgo2 | GTACAGGCAGTAAACCAG(T) | 20 | 46.5 | 739-758 | U91638 | |
| L. hackeliae | Lha | AGGGTGACGGCGCTAA(T) | 16 | 49.3 | 690-705 | U92207 |
| L. jordanis | Ljo | AAGCATTCTTAAACGCAAAC(T) | 20 | 46.9 | 767-786 | U92209 |
| L. lansingensis | Lla1 | GTTACTCACAGAGCAGCAAA(T) | 20 | 45.3 | 630-649 | U92210 |
| Lla2 | AAACGCAGCAACGCCTACT(T) | 19 | 52.6 | 447-465 | U92210 | |
| L. longbeachae | Ll | ACTGGTACCTTGATTGATG(T) | 19 | 41.5 | 1004-1022 | X83036 |
| L. maceachernii | Lma1 | ACAATAAGGCAAAAGGAG(T) | 18 | 41.6 | 680-697 | U92211 |
| Lma2 | TCATTGAGCGCGGTGAT(T) | 17 | 50.1 | 767-783 | U92211 | |
| L. micdadei | Lmi1 | AGCTTTCCTTAACGAAAA(T) | 18 | 41.9 | 1039-1056 | S62141 |
| Lmi2 | CACCGGCAAGCTGATTG(T) | 17 | 50.3 | 1165-1181 | S62141 | |
| L. oakridgensis | Loa1 | AATGGTTCAAGGGTTGCA(T) | 18 | 48.2 | 284-301 | U92214 |
| Loa2 | CTGGCTCCGTTTGGGA(T) | 18 | 49.4 | 685-700 | U92214 | |
| L. pneumophila | Lp1 | AAACAAGCCAGGCGTT(T) | 16 | 45.6 | 329-344 | AF022334 |
| Lp2 | CAATTGGCTTTAACCGAACAA(T) | 21 | 52.2 | 189-208 | AF022334 | |
| L. sainthelensi | Lsai | ACTGGTGCGAAACCCG(T) | 16 | 49.3 | 853-868 | U91219 |
| L. tucsonensis | Lagtwc | CCTTCAGACTTAGCTTAT(T) | 18 | 35.1 | 1072-1089 | U91607 |
| Ltu | (T)TCAAAATCCGGCGTAGT | 17 | 45.8 | 804-820 | U92224 | |
| L. wadsworthii | Lagtwc | CCTTCAGACTTAGCTTAT(T) | 18 | 35.1 | 1072-1089 | U91607 |
| Lwad1 | CAGTAAGACAGATACTGTTACTGT(T) | 24 | 42.2 | 740-756 | U92225 | |
| Lwad2 | AGGCGATTCATTCTTA(T) | 16 | 36.6 | 638-653 | U92225 | |
| Positive control | PC | CARGTNATHCCNGGNTGGACHGA(T) | 23 | 60.5 | 510-532 | AF022334 |
a
(T), additional bases of thymine (T) were added to the 5′ or 3′ end of the probe. Fifteen, 10, or 5 thymine bases were added to probes that had a Tm of ≤40°C, ≤45°C, or >45°C, respectively. The positive control probe contained 15 thymine bases at the 3′ end.
b
The location of the probe is indicated by the nucleotide numbers of the mip gene, unless otherwise indicated.
c
Group-specific probe.
d
A single mismatch base was intentionally incorporated into the probe to avoid cross-hybridization by other Legionella spp.