TABLE 2.
Sample no. | Amt of frozen plasma RNA (log10 copies/ml) | % Similarity score for frozen vs. dried plasmaa
|
|||
---|---|---|---|---|---|
RAMs
|
Polymorphic fingerprint
|
||||
Nucleotides (n = 165) | Amino acids (n = 55) | Nucleotides (n = 924) | Amino acids (n = 308) | ||
1 | 3.49 | 99.4 | 100 | 99.9 | 100 |
2 | 3.59 | 99.7 | 100 | 98.3 | 97.6 |
3 | 4.14 | 100 | 100 | 99.7 | 100 |
4 | 4.23 | 99.4 | 99.1 | 99.7 | 99.4 |
5 | 4.34 | 100 | 100 | 99.8 | 99.8 |
6 | 4.40 | 100 | 100 | 99.6 | 100 |
7 | 4.45 | 100 | 100 | 100 | 100 |
8 | 4.50 | 99.4 | 99.1 | 99.4 | 99.4 |
9 | 4.59 | 100 | 100 | 99.6 | 99.7 |
10 | 4.62 | 99.7 | 100 | 99.7 | 99.8 |
11 | 4.68 | 99.7 | 100 | 99.7 | 100 |
12 | 4.81 | 100 | 100 | 100 | 100 |
13 | 4.84 | 100 | 100 | 99.5 | 100 |
14 | 4.86 | 100 | 100 | 99.7 | 100 |
15 | 4.94 | 100 | 100 | 99.5 | 100 |
16 | 4.97 | 99.7 | 99.1 | 99.7 | 99.7 |
17 | 4.99 | 100 | 100 | 99.9 | 100 |
18 | 5.00 | 100 | 100 | 100 | 100 |
19 | 5.26 | 100 | 100 | 99.9 | 100 |
20 | 5.41 | 100 | 100 | 99.9 | 100 |
Mean score | 99.9 | 99.9 | 99.7 | 99.8 |
Specimens were air dried and stored at ambient temperature for 4 to 7 days prior to testing. TruGene HIV-1 genotyping kit-derived real-time first-pass sequences were used as the “gold standard” reference sequence for comparison to dried ST specimens. Stringency of scoring includes IUB base codes for mixture detection.