Skip to main content
. 2009 Mar 25;47(5):1491–1496. doi: 10.1128/JCM.02354-08

TABLE 2.

HIV-1 genotypic resistance test results comparing frozen- and dried-plasma specimens

Sample no. Amt of frozen plasma RNA (log10 copies/ml) % Similarity score for frozen vs. dried plasmaa
RAMs
Polymorphic fingerprint
Nucleotides (n = 165) Amino acids (n = 55) Nucleotides (n = 924) Amino acids (n = 308)
1 3.49 99.4 100 99.9 100
2 3.59 99.7 100 98.3 97.6
3 4.14 100 100 99.7 100
4 4.23 99.4 99.1 99.7 99.4
5 4.34 100 100 99.8 99.8
6 4.40 100 100 99.6 100
7 4.45 100 100 100 100
8 4.50 99.4 99.1 99.4 99.4
9 4.59 100 100 99.6 99.7
10 4.62 99.7 100 99.7 99.8
11 4.68 99.7 100 99.7 100
12 4.81 100 100 100 100
13 4.84 100 100 99.5 100
14 4.86 100 100 99.7 100
15 4.94 100 100 99.5 100
16 4.97 99.7 99.1 99.7 99.7
17 4.99 100 100 99.9 100
18 5.00 100 100 100 100
19 5.26 100 100 99.9 100
20 5.41 100 100 99.9 100
Mean score 99.9 99.9 99.7 99.8
a

Specimens were air dried and stored at ambient temperature for 4 to 7 days prior to testing. TruGene HIV-1 genotyping kit-derived real-time first-pass sequences were used as the “gold standard” reference sequence for comparison to dried ST specimens. Stringency of scoring includes IUB base codes for mixture detection.