TABLE 2.
Primers used for genotyping of VZV isolates
| ORF (restriction site) | Primer sensea | Primer sequence (5′→3′) | Genome positionb | Reference |
|---|---|---|---|---|
| ORF 1 | F | TCA GCT GGC TTT TCT AAG AAT TCG | 427-450 | 2 |
| R | TAT TTT TGG GAT CCG CAA TGT C | 932-911 | ||
| ORF 21 | F | TGG CGC GGT TTA AAT GAA TTG A | 33497-33518 | 2 |
| R | CAC GTG TAG CTC CAA AAA CCT AGG | 33999-33976 | ||
| ORF 54 (BglI) | F | CGT AAT GCA TAA CAG GCC AAC AC | 95005-95027 | 2 |
| R | AAA CCT GGC GTC AAA CAT TAC A | 95501-95497 | ||
| ORF 22 | F | GGG TTT TGT ATG AGC GTT GG | 37837-37856 | 13 |
| R | CCC CCG AGG TTC GTA ATA TC | 38283-38264 | ||
| ORF 38 (PstI) | F | AAG TTT CAG CCA ACG TGC CAA TAA A | 69060-69084 | 7 |
| R | AGA CGC GCT TAA CGG AAG TAA CG | 69706-69684 | ||
| ORF 62 (SmaI) | F | TTC CCA CCG CGG CAC AAA CA | 106036-106055 | 14 |
| R | GGT TGC TGG TGT TGG ACG CG | 106303-106284 | ||
| R5 | F | GGC AAA TAC TTA GAC CGT TTT | 101992-102012 | 6 |
| R | TAA TGG ACT TTT AAT GGA TTG | 102350-102329 |
a
F, forward; R, reverse.
b
Based on the sequence with GenBank accession number NC_001348.