TABLE 1.

Influenza virus matrix gene M2 primers

Method	Amplification primer		Pyrosequencing primer
	Name	Sequence	Name	Sequence
Pyrosequencing	M2_F	5′-CAGATGCARCGATTCAGTGa	M2seqF	5′-CAGATGCAGCGATTCAGTGa
	M2_R Biotin	5′-biotin-AGTAGAAACAAGGTAGTTTTTTACTCa
	M2_F Biotin	5′-biotin-CAGATGCAGCGATTCAAGTGb	M2seqR	5′-AATCCACAATATCAAGTGCA
	M2_R	5′-AGTAGAAACAAGGTAGTTTTTTACTCa
Dideoxy	DDseqM2_F	5′-AGCACTACAGCTAAGGCTATGGc
	M2_R	5′-AGTAGAAACAAGGTAGTTTTTTACTCa,c

^aFrom reference 4.

^bModified from reference 4 by the addition of biotin, the substitution of G for R, and the insertion of an additional A (changes in boldface type).

^cPrimer also used for dideoxy sequencing.