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Evaluation of probe BRACHY, specific for Brachyspira spp. Fixed bacteria from cultures of B. pilosicoli, E. faecium, B. garinii, T. denticola, and L. biflexa were simultaneously hybridized with BRACHYCy3 (orange) and EUB338Cy5 (magenta). Left and right panels in each row show identical microscopic fields with filter sets for Cy3 and Cy5, respectively. BRACHY showed specific hybridization, giving a positive signal solely with B. pilosicoli.
