FIG. 2.

Overrepresentation and underrepresentation of segments of the NCRR in plasma and urine samples as a result of insertions and deletions. For each clinical sample, the insertions for each cloned mrNCRRs were removed in silico, and all that remained of each sequence was aligned to its respective WWL1 or WWL2 archetypes, allowing for large gaps (Sequencher program). The partial or complete segments contained in the insertions that had been removed in silico were then added to the alignment. The number of nucleotides at each position was determined and then normalized as a function of the number of clones that were analyzed for that clinical sample. The shaded areas represent the average normalized value for each nucleotide position for all plasma samples and all urine samples. A value of >0 indicates overrepresentation, a value <0 indicates underrepresentation, and a value of 0 indicates that either there were no insertions or deletions at this position or that the number of insertions was compensated for by the number of deletions.