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. 2009 Feb 13;191(11):3451–3461. doi: 10.1128/JB.01539-08

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FIG. 1. — ECP on EPEC as shown by electron microscopy and immunogold labeling. (A) Electron micrograph of E2348/69 grown in DMEM displaying abundant peritrichous fine pili (ECP) and flagella (Fla). (B and C) Immunogold labeling of ECP on E2348/69 with anti-ECP antibodies and preimmune serum, respectively. (D) E2348/69 grown in LB medium showing no pili. (E) E2348/69ΔecpA mutant showing no ECP. (F) E2348/69ΔecpA(pMR13) with restored ECP production. Scale bars, 100 nm. (G) Detection of EcpA in normalized HCl-treated whole-cell extracts.