TABLE 1.
Strains and plasmids used
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| EPEC strains | ||
| E2348/69 | O127:H6 (prototype wild type) | 28 |
| E2348/69ΔecpA | ecpA::km mutant | This study |
| E2348/69ΔecpA(pMR13) | ΔecpA complemented with pMR13 | This study |
| 31-6-1(1) | E2348/69 ΔbfpA | 7 |
| 31-6-1(1)ΔecpA | E2348/69 ΔbfpA ΔecpA::km | This study |
| UMD872 | E2348/69 ΔespA | 20 |
| UMD872ΔecpA | E2348/69 ΔespA ΔecpA::km | This study |
| JPN15.96 | EAF plasmid− intimin− | 17 |
| JPN15.96ΔecpA | JPN15.96 ΔecpA::km | This study |
| HB101 | E. coli B-K-12 hybrid | Lab collection |
| HB101(pCVD462) | HB101 carrying pCVD462 | This study |
| HB101(pMAR7) | HB101 carrying pMAR7 | This study |
| HB101(pMAT9) | HB101 carrying pMAT9 | This study |
| HB101(pSE380) | HB101 carrying pSE380 | This study |
| Plasmids | ||
| pKD46 | Red recombinase system plasmid | 6 |
| pKD4 | kan cassette template plasmid | 6 |
| pMR13 | ecpA cloned in pGEM-T | 36 |
| pCVD462 | LEE region of EPEC in pCVD551 | 29 |
| pMAR7 | EAF plasmid of EPEC E2348/69 | 1 |
| pMAT9 | ecpAB in pSE380 | 34 |
| pSE380 | Expression vector, trc promoter | Invitrogen |