TABLE 1.
Strains used in this work
| Strain | Genotype | Parental strain | Mutated ORF(s) | Plasmid used for transformation | Antibiotic resistance |
|---|---|---|---|---|---|
| WT | Synechocystis sp. strain PCC 6803 | ||||
| SARS4 | arsC::C.K1 | WT | slr0946 | pARSC2 | Km |
| SARS5 | arsR::C.C1 | WT | sll1957 | pARSR2 | Cm |
| SARS6 | arsI1::SpΩ | WT | sll5104 | pARSI2 | Sp |
| SARS7 | arsI2::SpΩ | WT | slr6037 | pARSI2 | Sp |
| SARS8 | ΔarsI2::SpΩ | WT | slr6037 | pARSI8 | Sp |
| SARS9 | arsI1::SpΩ ΔarsI2::C.C1 | SARS6 | slr6037, sll5104 | pARSI9 | Sp Cm |
| SARS10 | arsC::C.K1 arsI1::C.C1 | SARS4 | slr0946, sll5104 | pARSI4 | Km Cm |
| SARS11 | arsC::C.K1 ΔarsI2::SpΩ | SARS4 | slr0946, slr6037 | pARSI8 | Km Sp |
| SARS12 | arsC::C.K1 arsI1::C.C1 ΔarsI2::SpΩ | SARS10 | slr0946, slr6037, sll5104 | pARSI8 | Km Cm Sp |
| SGRXA | grxA::C.C1 | WT | ssr2061 | pGRXA2 | Cm |
| SGRXB | grxB::SpΩ | WT | slr1562 | pGRXB2 | Sp |
| SGRXC | grxC::C.K1 | WT | slr1846 | pGRXC2 | Km |
| SGRXAB | grxA::C.C1 grxB::SpΩ | SGRXA | ssr2061, slr1562 | pGRXB2 | Cm Sp |
| SGRXAC | grxA::C.C1 grxC::C.K1 | SGRXA | ssr2061, slr1846 | pGRXC2 | Cm Km |
| SGRXBC | grxB::SpΩ grxC::C.K1 | SGRXB | slr1562, slr1846 | pGRXC2 | Sp Km |
| SGRXABC | grxA::C.C1 grxB::SpΩ grxC::C.K1 | SGRXAB | ssr2061, slr1562, slr1846 | pGRX2C | Cm Sp Km |