TABLE 3.
Primers used to generate null mutations in different exonuclease genes
| Gene | Primera | Sequence |
|---|---|---|
| xonA | H1P1 | ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTGTGTAGGCTGGAGCTGCTTC |
| H2P2 | GACAATCTCTTCCGCGTACTGCCAAAGTGCTTTTAACAGCATATGAATATCCTCCTTAG | |
| CHECK | CCAGGACCCGGCATGAATCTCTG | |
| recJ | H1P1 | AAACAACAGATACAACTTCGTCGCCGTGAAGTCGATGAAAGTGTAGGCTGGAGCTGCTTC |
| H2P2 | TGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCATATGAATATCCTCCTTAG | |
| CHECK | GGGATCACCGTGCGTTATCTTGC | |
| xseA | H1P1 | TTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCGTGTAGGCTGGAGCTGCTTC |
| H2P2 | CTTTCTATCCAGCCGTCTTCCAGACGTGTGGTTAGCATTTCATATGAATATCCTCCTTAG | |
| CHECK | CGTTACGCTCGGTCAGTTCTTTCAC | |
| exoX | H1P1 | ATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGTGTAGGCTGGAGCTGCTTC |
| H2P2 | AGTATTTTCCAGATAATGTTTCAGTGTTAAACGCAGCTCCATATGAATATCCTCCTTAG | |
| CHECK | GGATCTACCATCAGAGTCATGGTGC |
a
H1 corresponds to approximately the first 12 codons and H2 corresponds to the last 12 codons of each particular gene. P1 and P2 are underlined and correspond to the regions flanking the antibiotic resistance cassette located on the pKD3 and pKD4 plasmids (8).