TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| ATCC 43037 | T. forsythia | 45 |
| DH5α | E. coli K-12 cloning host | Invitrogen |
| α-Select | E. coli K-12 cloning host | Bioline |
| BL21(DE3) | E. coli B expression host | Novagen |
| KCL116 | BL21(DE3)/pET30 | This study |
| KCL117 | BL21(DE3)/pET30::nanH | This study |
| KCL120 | BL21(DE3)/pET30::siaHI | This study |
| Plasmids | ||
| pCR4-Topo | PCR cloning vector; Kmr Ampr | Invitrogen |
| pET30a, -b, and -c | pET30 expression vectors a, b, and c containing an inducible T7 promoter; Kmr | Novagen |
| pKCL175 | pCR4-Topo containing nanH from T. forsythia, amplified using primers P265 and P266 | This study |
| pKCL191 | pET30c containing nanH from pKCL175 cloned into the BamHI site | This study |
| pKCL202 | pET30b containing siaHI from T. forsythia, amplified using the primers P291 and P292 | This study |
a
Ampr, ampicillin resistance; Kmr, kanamycin resistance.