TABLE 3.
Genomic islanda | Island size (kb) | Cochallengeb
|
Independent challengec
|
||||
---|---|---|---|---|---|---|---|
No. of miced |
P valuese
|
No. of mice |
P values
|
||||
Bladder | Kidneys | Bladder | Kidneys | ||||
PAI-CFT073-aspV(PAI III) | 100 | 20 | 0.0139 | <0.0001 | 11 | 0.8201 | 0.3692 |
φ-CFT073-b0847 | 33 | 8 | 0.2969 | 0.0781 | ND | ND | ND |
PAI-CFT073-serX | 113 | 8 | 0.6406 | 0.1563 | ND | ND | ND |
φ-CFT073-potB | 44 | 9 | 0.9102 | 0.0781 | ND | ND | ND |
PAI-CFT073-asnT(HPI) | 32 | 10 | 0.1641 | 0.0078 | 10 | 0.8793 | 0.3211 |
GI-CFT073-asnW | 54 | 13 | 0.7869 | 0.5879 | 13 | >0.9999 | 0.3622 |
GI-CFT073-cobU | 44 | 10 | 0.4922 | 0.4922 | ND | ND | ND |
PAI-CFT073-metV | 32 | 16f | 0.0020 | 0.0003 | 10 | 0.0668 | 0.0011 |
GI-CFT073-selC | 68 | 13 | 0.2163 | 0.4648 | 13 | 0.6810 | 0.6139 |
PAI-CFT073-pheV (PAI I) (123 kb) and PAI-CFT073-pheU (PAI II) (52 kb) mutants were not tested in vivo, as these PAIs have already been published.
In cochallenge infection experiments, mice were inoculated with a 1:1 ratio of standardized cultures of wild-type CFT073 and a deletion mutant.
In independent challenge experiments, mice were inoculated separately with standardized cultures of either wild-type CFT073 or the deletion mutant. ND, not determined.
Number of mice with detectable colonization in bladder and/or kidneys.
P values comparing the ability of the mutants with the indicated genomic island to colonize the bladder and kidneys of mice shown in bold type were statistically significant (P < 0.05). P values for cochallenge infections were determined using the Wilcoxon matched-pair test. P values for independent challenges were determined using the Mann-Whitney test.
Sixteen mice were used in the PAI-metV cochallenge experiments, although only 14 data points were usable for the bladder data.