TABLE 2.
Straina | ADK activity (pmol AMP min−1 mg protein−1)b | Adenosine content (nmol g−1 [dry wt] cells)b | ATP content (μmol g−1 [dry wt] cells)b at:
|
||
---|---|---|---|---|---|
12 h | 24 h | 36 h | |||
8004 (wild type) | 59.1 ± 2.2 a | 14.3 ± 2.1 a | 3.4 ± 0.18 a | 2.52 ± 0.12 a | 1.75 ± 0.14 a |
0690pk (adkXcc mutant) | 5.5 ± 0.8 b | 142 ± 17 b | 2.82 ± 0.13 c | 2.01 ± 0.15 c | 1.35 ± 0.11 c |
C0690pk (complemented mutant) | 77 ± 3.8 a | 12.6 ± 3.2 a | 3.36 ± 0.15 a | 2.45 ± 0.18 a | 1.83 ± 0.15 a |
Strains were cultured in the nutrient-rich complex medium NYG.
Data presented are the means ± standard deviations of triplicate measurements from a representative experiment, and similar results were obtained in two other independent experiments. Different letters a and b indicate significant differences at a P value of 0.01 (t test); a and c indicate significant differences at a P value of 0.05.