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. 2009 Mar 27;191(11):3685–3697. doi: 10.1128/JB.00202-09

FIG. 2.

FIG. 2.

Determination of the transcription start sites of the yetM and yetL genes by primer extension analysis. Total RNAs of strains 168 (wild type) (lane 1) and YETLd (yetL disruptant) (lane 2) used for determination of the transcription start site of yetM (left panel) and total RNAs of FU1035 (yetL+) (lane 1) and FU1038 (ΔyetL::tet) (lane 2) used for determination of the transcription start site of yetL (right panel) were prepared and used for the reverse transcription reaction to generate the runoff cDNAs. Lanes G, A, T, and C contained the products of the dideoxy sequencing reactions obtained with the same primer that was used for reverse transcription. The runoff cDNAs are each indicated by an arrow. The partial nucleotide sequences of the coding strands corresponding to the ladders are shown; the “−35” and “−10” sequences are underlined, and the transcription start sites (+1) and the SD sequence are enclosed in boxes.