FIG. 1.
Effects of RpoS on P. putida survival under carbon starvation conditions and frequency of accumulation of 1-bp deletions. (A) Viability of P. putida WT strain PaW85 and its rpoS-deficient derivative on phenol minimal plates. (B) Accumulation of Phe+ revertants on phenol minimal plates in P. putida WT strain PaW85 and its rpoS-deficient derivative carrying plasmid pKTpheA56+A. About 5 × 108 P. putida cells from independent cultures grown overnight in liquid M9 medium were plated onto phenol minimal plates. Data for at least five parallel experiments are presented. In all cases, means ± standard deviations (error bars) for 10 plates calculated per 1 × 109 cells are shown.