TABLE 3.
Amounts of superoxide in lysates and supernatants of cells of the P. putida WT strain and its rpoS-deficient derivative
| Strain (day of sampling) | Amt of superoxide/ plate | Amt of superoxide/106 CFUc |
|---|---|---|
| WT strain | ||
| Lysate Ia (2) | 35,956 ± 5,740 | 32.0 ± 10.1 |
| Supernatant I (2) | 11,509 ± 1,501 | 11.2 ± 2.1 |
| Supernatant II (2) | 561 ± 251 | 0.8 ± 0.07 |
| Lysate IIb (2) | 28,938 ± 4,772 | 33.0 ± 22.8 |
| Lysate Ia (8) | 32,677 ± 4,649 | 42.9 ± 9.8 |
| Supernatant I (8) | 14,910 ± 2,722 | 21.0 ± 6.2 |
| Supernatant II (8) | 1,829 ± 452 | 3.7 ± 1.0 |
| Lysate IIb (8) | 20,101 ± 1,888 | 39.7 ± 13.8 |
| RpoS-deficient strain | ||
| Lysate Ia (2) | 82,677 ± 11,407 | 111 ± 13.3 |
| Supernatant I (2) | 27,033 ± 2,780 | 43.8 ± 8.3 |
| Supernatant II (2) | 3,165 ± 1,324 | 7.4 ± 0.4 |
| Lysate IIb (2) | 36,667 ± 4,598 | 62.9 ± 12.1 |
| Lysate Ia (8) | 73,189 ± 11,972 | 8,832 ± 1,599 |
| Supernatant I (8) | 32,812 ± 6,370 | 4,302 ± 803 |
| Supernatant II (8) | 11,177 ± 5,446 | 1,861 ± 994 |
| Lysate IIb (8) | 28,103 ± 4,324 | 4,481 ± 1,062 |
a
Cells from agar plates sonicated within their resuspension buffer (see preparation of lysate I in Materials and Methods).
b
Cells sonicated after washing with M9 (see preparation of lysate II in Materials and Methods).
c
Relative amount of SO per 1 × 106 CFU in 1 ml reaction mixture; values for supernatant I and supernatant II are given per 1 × 106 CFU in 1 ml of cell suspension before and after washing of cells, respectively.