FIG. 1.

Phenotypes of V. cholerae tryptophanase mutants. (A) Biofilm accumulation of wild-type (WT) SIO and TP strains and their corresponding tnaA::Tn5 mutants S1101 and T1101 grown in LB medium (gray bars) and LB medium supplemented with indole (350 μM; white bars). (B) Confocal micrographs of the biofilms of S4100 and S4101 grown in LB without indole or with 500 μM indole (+I) for 6 h under static conditions. The top panels are the x-y axis, and bottom panels are the z axis. (C) Measure of the auto-aggregation phenotype of SIO and S1101 grown in LB medium with 500 μM indole (white bars) or without (gray bars). Error bars represent 1 standard deviation from the mean of three biological replicates for each strain.