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Two classes of RSCVs have been isolated based on complementation patterns. Class A RSCVs, but not class B, RSCVs are complemented by supplying wspF in trans (pWspF). Both classes of RSCVs are suppressed by depleting c-di-GMP in the cells by expressing the phosphodiesterase encoded by PA2133 (pPA2133). The images show colony morphology, while the graphs show the adherence in a microtiter dish binding assay.
