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Our clinical and laboratory RSCVs both display elevated c-di-GMP levels: 2D TLC of cells labeled with 32PPi. Cells were extracted and hydrolyzed following labeling, and the labeled nucleotide fraction was chromatgraphed as previously described (17). Both wild-type strains (PAO1 and CF39wt) produced little measurable c-di-GMP, while the two RSCVs (MJK8 and CF39s) produced a dark, radiolabeled spot that migrated with c-di-GMP (indicated by an arrow).
